| RNASE6

Ribonuclease A family member k6 is a protein that is encoded by the RNASE6 gene.The protein encoded by this gene is a member of the ribonuclease A superfamily and functions in the urinary tract. The protein has broad-spectrum antimicrobial activity against pathogenic bacteria.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.02	1.4	0.86	0.59	0.38	0.26	0.18	0.08
Corrected OD	1.94	1.32	0.78	0.51	0.3	0.18	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human RNASE6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	92-105	102
Cell Lysates(n=8)	96-108	101

 

| Linearity

Samples were spiked with high concentrations of Human RNASE6 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	85-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	96	98	108